Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black-streaked dwarf virus. Sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Rice black-streaked dwarf virus: Its properties, morphology and intracellular localization. Epidemiological studies on the rice black-streaked dwarf virus in Kanto-Tosan district, Japan. RBSDV SELEX aptamers emulsion PCR rice black-streaked dwarf virus. In the first SELEX round, the entire cell-bound ssDNA pool (50 L) was subject to preparative PCR, whereas in subsequent rounds approximately 15 L of the cell-bound ssDNA pool was amplified by. This is the first report of aptamers being selected for detection of a rice virus capsid protein. The presence of up to 10 15 different strands with undefined random regions can lead to several issues such as by-product formation in the PCR. Droplet-based digital PCR system for detection of single-cell level of foodborne pathogens. These data show that the selected aptamers can be used for the detection of RBSDV P10 protein in vitro and in vivo. SELEX libraries typically comprise up to 100 nucleotides in length, containing a random region flanked by two constant primer-binding sites, which are used for PCR amplification of the library. Partitioning can also allow for more robust. All three DNA aptamers can be used to detect RBSDV P10 protein by immunofluorescent labeling in the midgut of RBSDV-infected SBPH. The specificity of these three DNA aptamers was tested through detection of the P10 protein using an enzyme-linked oligonucleotide assay (ELONA) and aptamer-based dot-blot ELISA. In order to provide a stable and cost-effective detection probe, in this study we selected three DNA aptamers (R3, R5 and R11) by an optimized, standardized and time saving emulsion PCR-based SELEX, for the detection of RBSDV outer-shell P10 protein for in situ localization studies in the midgut of SBPH. RBSDV is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus Fallén) in a persistent manner. Rice black-streaked dwarf virus (RBSDV), classified under the Reoviridae, Fijivirus genus, caused an epidemic in the eastern provinces of China and other East Asian countries and resulted in severe yield loss in rice and wheat production.
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